Full-length transcriptome analysis provides insights into larval shell formation in Mulinia lateralis

Mollusca is the second largest animal phylum and represents one of most evolutionarily successful groups. Mulinia lateralis , a small bivalve, promising model organism to facilitate studies mollusc development. However, because lack published genomic transcriptomic resources, integrated research on formation larval shells in this species, which representative developmental process molluscs great importance for larva survival, hindered. In study, blastula, gastrula, trochophore larva, D-shaped M. were utilized generating comprehensive full-length transcriptome through Pacific BioSciences (PacBio) isoform sequencing (Iso-seq) Illumina RNA-Seq. A total 238,919 transcripts with an average length 3,267 bp 121,424 annotated genes obtained. RNA-Seq data analysis showed that 4,512, 10,637, 17,829 differentially expressed (DEGs) obtained between two adjacent stages. Functional annotation enrichment revealed specific function shell biomineralization during different Twelve may be involved identified, including insoluble matrix protein-encoding gene 1 ( ISMP1 ), ISMP2 ISMP5 chitin synthase tyrosinase chitin-binding protein collagen pu14 deposition, carbonic anhydrase solute carrier family 4 member 8 slc4a8 EF-hand calmodulin coding C-2442 participated ion transportation. addition, calcium binding function, signaling pathway, endocrine other factor-regulated reabsorption pathways significantly enriched. Weighted correlation network (WGCNA) identified modules related formation, ring finger 41 rnf41 ) key hub process. Moreover, it could implied transport occurs earlier than deposition matrix. This work provided clear view will valuable elucidating mechanisms as well processes molluscs.